DNA probe to the T-cell receptor beta-chain constant region.
Whole blood, bone marrow, lymph node, or other tissue.
7 mL whole blood or 2 cc bone marrow or 0.5-1 g tissue.
3 mL whole blood.
Whole blood or bone marrow in lavender-stopper (EDTA) tubes, lymph node or tissue in lymph node transport bottle.
The specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the test request form.
Maintain specimen at room temperature.
Specimen frozen; specimen in formalin or another fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days.
Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T-lymphocytes.
Southern blot hybridization.